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Thông tin chi tiết sản phẩm:
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| Nhanh: | ＜30 phút | Phục hồi cao: | 80-105% |
|---|---|---|---|
| Thời gian phát hiện: | 1 giờ | Nhiệt độ lưu trữ: | 2-8 ° C. |
| Đặc điểm kỹ thuật: | 96T | Khoảng thời gian tuyến tính: | 0,5 ng/ml |
Saxitoxin (PSP) ELISA Test Kit
CH 98013
Saxitoxin (PSP) ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Saxitoxin in mussels and water samples. Saxitoxin, the principal component of paralytic shellfish poisons (PSP), blocks the sodium ion channels in nerve and muscle membranes.The lethal dose in humans is 1 to 3 mg. Numbness and respiratory paralysis can occur after oral ingestion of as little as 0.5 - 1.0mg saxitoxin. Saxitoxin residues can be determined by either GC-MS or HPLC. However, enzyme immunoassays (ELISA) show considerable advantages as it is faster and more sensitive.
Saxitoxin (PSP) ELISA Test Kit enables international and government regulatory agencies, food manufacturers and processors, as well as quality assurance organizations, to detect saxitoxin in shellfish matrices and to satisfy customer concerns about food safety.
§ High recovery (80%-105%), rapid (30 minutes), and cost-effective extraction methods
§ High sensitivity (0.5 ng/g or ppb) and low detection limit (100 ppb for mussel samples ).
§ High reproducibility.
§ A quick ELISA assay(less than 1 hours regardless of number of samples).
| Kit Contents | Amount | Storage |
| Second antibody-Coated Plate | 1 x 96-well Plate(8 wells x 12 strips) | 2-8°C |
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Saxitoxin (PSP) Standards: Negative control 0.5 ng/mL 1.5 ng/mL 4.5 ng/mL 13.5ng/mL
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1 mL
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-20°C |
| Antibody Solution | 8 ml | 2-8°C |
| Okadaic Acid-HRP Conjugate | 15 mL | |
| 5X Wash Solution | 50mL | |
| Stop Buffer | 7mL | |
| Color Solution | 7 mL | |
| Sample Diluent Concentrate (10X) | 17 mL |
Shellfish samples
1) Remove the shells and extract the meat. Homogenize the meat samples;
2) Weigh 10g of the homogenized samples into a 50mL centrifuge tube;
3) Add 20mL of 0.1M hydrochloric acid, vigorously shake and mix well, and heat in boiling water bath for 5 minutes;
4) After the samples cool down, centrifuge at 4000r/min at room temperature for 10 minutes;
5) Take 50μL of the supernatant and add it to 950μL of sample dilution solution, mix well;
6) Take 50μL of the mixed solution for detection.
Người liên hệ: Tina
Tel: +8615818799493
